Protein Production Services

Rodon Biologics provides small to large-scale protein expression and purification. Our protein production capabilities range from parallel screening of several constructs at small scale through process development optimization to manufacturing.

Starting from your gene sequence, recombinant vectors, hybridoma cells or stable cell lines, we produce small amounts of numerous molecules in parallel and scale up to produce larger amounts, depending on your needs. Our custom protein and custom protein services are tailored to meet specific research needs, ensuring personalized protein production services.

Selecting the appropriate expression system based on the protein's characteristics and intended use is crucial for optimal production and functionality.

We can use our platform production process, execute your protocol, or customize a process to meet your yield and quality requirements.

Our services include:

• Molecular Cloning

• Vector construction
• Customizing - e.g., inclusion of tags for purification, insertion of protease signal for tag removal, signal peptides for secretion

• Protein Expression

• Expression systems:
• Bacteria: E. coli
• Yeast: Saccharomyces cerevisiae, Pichia pastoris
• Mammalian cells: CHO and HEK293
• Transient production: from mg-gram quantities in shake flasks and bioreactors (10L working volume)
• Stable engineered cell lines: grams per liter in fed batch processes

• Protein Purification and Isolation

• Purification of soluble proteins (cytoplasm, periplasm, secreted)
• Purification of insoluble proteins from inclusion bodies, including refolding
• Chromatography (affinity, Ion-X, HIC, MMC, RP, membrane)
• Filtration (Depht, Micro, X-flow, viral)

• Quality Control

• Assays for concentration (A280, Dye binding assay and HPLC)
• Assays for purity (HPLC, gel electrophoresis, Western Blot, Endotoxin level and microbiological tests)
• ELISA and product specific functional assays
• Stability testing

Protein Expression Systems

Mammalian Transient and Stable Expression and purification

Mammalian expression systems are essential for producing recombinant proteins, particularly when post-translational modifications are necessary for functionality, such as in antibodies or multispecific antibodies. These systems, often combined with our expertise in protein engineering and antibody services, ensure optimal results. Our mammalian transient and stable expression and purification services are designed to deliver high-quality proteins for various research and therapeutic applications.

• Transient expression allows for rapid production of proteins in small to medium quantities, making it suitable for proof-of-concept studies and high throughput screening.
• Stable expression, on the other hand, is ideal for large-scale production, providing consistent and reliable yields of target proteins.

Our services accommodate a diverse array of molecules, including antibodies, enzymes, membrane proteins, and therapeutic proteins. We also specialize in the production of fusion proteins and proteins requiring complex post-translational modifications. Utilizing CHO and HEK293 cells, our mammalian expression systems ensure that the proteins produced maintain their native structure and function, crucial for downstream applications.

The purification process is equally critical, involving advanced techniques such as affinity chromatography, ion exchange, and size exclusion chromatography, to achieve the highest quality and purity of the expressed proteins. Our extensive range of services ensures that proteins are expressed and thoroughly purified to meet the precise needs of your research or therapeutic projects.

E. coli Recombinant Protein Production

Our E. coli protein expression services are designed to optimize expression conditions and achieve high protein solubility and yield. We utilize advanced techniques such as codon optimization and gene synthesis to enhance protein expression and ensure the production of high-quality proteins. Our services also include protein purification and quality control to deliver purified proteins that meet your research or industrial needs.

E. coli systems are particularly suitable for producing proteins that do not require complex post-translational modifications, making them an excellent choice for many research and development projects. Whether you need small-scale protein production for initial experiments or medium-scale manufacturing for research applications, our E. coli recombinant protein production services provide a reliable and versatile solution.

Expertise in Soluble, Periplasmatic, and Inclusion Body Proteins

At Rodon Biologics, our expertise extends to the production and purification of proteins in various forms, including soluble, periplasmatic, and inclusion bodies.

In cases where proteins are expressed as inclusion bodies, our team employs advanced refolding strategies to recover functional proteins. This involves solubilizing the inclusion bodies and carefully refolding the proteins to achieve their native structure. Our comprehensive approach ensures that whether the proteins are soluble, periplasmatic, or from inclusion bodies, they meet the highest quality standards for your research and industrial applications.

Yeast Cell Protein production

Yeast cells are an advantageous choice for protein production due to their ability to perform post-translational modifications, ease of genetic manipulation, and cost-effectiveness in large-scale production. These characteristics make yeast an ideal system for producing recombinant proteins, especially those requiring complex modifications for proper functionality.

Rodon Biologics offers protein expression services utilizing yeast cells. Our team has formulated strategies to enhance protein yield in Pichia pastoris and Saccharomyces cerevisiae by optimizing expression plasmids, refining culture conditions, and perfecting the extraction and purification processes for functional proteins.

Why Choose Our Protein Production Services

Rodon Biologics brings dedicated expertise and experience in handling a variety of protein types, including recombinant proteins and multispecific antibodies. Our diverse protein expression systems— mammalian, bacterial and yeast, and insect cells—are designed to optimize expression and purification processes efficiently.

We ensure superior outcomes through stringent quality control measures, providing cost-effective solutions tailored to your specific research needs. Our extensive experience spans a wide range of molecular types:

Discover how our expertise at Rodon Biologics can enhance your protein production projects with tailored expression and purification services for both research and commercial applications. Watch this video to gain further insights into our services.