Protein Engineering Services

Protein engineering aims at obtaining improved molecules to enhance clinical efficiency, manufacturability, and reduce the immunogenicity of a promising clinic candidate. This is achieved by using genetic engineering and recombinant DNA technology to change their amino acid sequences. By changing the amino acid sequence of a protein, scientists can improve its stability, specificity or catalytic activity to get new or improved functions.

Rodon's Methods of Rational Protein Engineering

At Rodon, protein engineering uses a range of methods including rational protein engineering, site-directed mutagenesis and semi-rational design. Each method uses different ways to modify protein sequences and improve their functions. The choice of method depends on the application and the protein structure which determines the protein’s function and properties. Understanding this relationship is key to protein engineering. Rodon uses tools that predict a protein’s structure from its amino acid sequence to give us insights to design proteins with specific functions.

Method	Description
Rational Protein Engineering	Uses computational models and structural information to design proteins with specific functions
Semi-Rational Design	Combines computational tools with experimental approaches to guide mutation introduction
Site-Directed Mutagenesis	Specific amino acid substitutions are made to alter protein functions
DNA Shuffling	Recombines segments of DNA to create new protein variants

Our Expertise

Custom Protein Engineering

Applying advanced techniques like DNA shuffling and computational modeling, we develop proteins with enhanced thermal stability, enzymatic activity, substrate specificity, and more.

End-to-End Services

From gene synthesis and protein expression to purification and detailed characterization, we support every stage of your project. Our integrated approach means high quality, fast and project management.

Our Services on Protein Engineering

• Structure and Stability Optimization: Enhance protein folding, solubility, and thermal stability
• Activity Enhancement: Boost enzymatic efficiency, binding affinity, or catalytic activity.
• De Novo Design: Design novel proteins tailored to your research needs.
• Fusion Proteins: Create recombinant fusion proteins through genetic engineering of a fusion gene

Different Antibody Multispecific Formats and Antibody Fragments

Multispecific antibodies are engineered proteins designed to bind simultaneously to multiple targets, enhancing therapeutic efficacy and specificity. These multispecific formats are particularly useful in complex diseases like cancer where targeting multiple pathways can lead to better treatment outcomes. Developing these complex molecules requires deep understanding of protein engineering techniques including precise amino acid substitutions and advanced computational protein design to ensure optimal binding affinity and stability.

Antibody fragments, on the other hand, are smaller portions of antibodies that retain the ability to bind to antigens. Common fragments include single-chain variable fragments (scFv), antigen-binding fragments (Fab), and dimeric Fab2. These fragments offer advantages such as improved tissue penetration, reduced immunogenicity, and easier manufacturing, making them ideal for therapeutic and diagnostic applications. By using these advanced formats and fragments protein engineering is pushing the boundaries of what is possible in medicine and offering new hope for many diseases.

Our Services on Antibody Engineering

• Humanization & Affinity Maturation: Convert non-human antibodies into human-compatible versions and enhance their binding affinity.
• Fc Engineering: Modify Fc regions for improved effector functions or pharmacokinetics.
• Bispecific and Multispecific Antibodies: Design and construct antibodies capable of targeting multiple antigens.
• Custom Screening and Validation; High-throughput screening for the best variants, functional and stability testing to ensure quality and performance.

Why Choose Us?

• Proven Expertise: With over a decade of experience in protein engineering, our team has tackled challenges across various domains, including biopharmaceuticals and industrial proteins.
• Custom Solutions: No two projects are the same. We adapt our processes to meet your specific needs.
• Innovative Technology: By combining in silico predictions with experimental testing we deliver faster and with fewer iterations.

Learn more about Rodon Biologics' protein engineering project by watching our detailed video here.

Ready to elevate your protein engineering? Let us help you discover and innovate. Contact us today to talk about your project and how we can work for you.